Among all cell systems, iPSCs provide a uniquely powerful platform for gene editing. Unlike primary cells, iPSCs are a virtually unlimited resource, capable of indefinite expansion while retaining genetic integrity. This abundance makes them especially suitable for precise gene modifications using CRISPR/Cas9 or other editing tools, with a high degree of efficiency and reproducibility. Once edited, a single iPSC line can be differentiated into multiple disease-relevant cell types—such as neurons, cardiomyocytes, hepatocytes, or immune cells—allowing researchers to investigate the effects of a specific genetic alteration across different tissues of the same genetic background. This ability to derive multiple functional cell types from one edited iPSC line not only ensures consistency and comparability in experiments, but also reduces variability and cost. Gene editing in iPSCs is therefore indispensable for disease modeling, drug screening, functional genomics, and the development of personalized therapeutic strategies.
